Figure 4
![Figure 4](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1472-6807-9-1/MediaObjects/12900_2008_Article_221_Fig4_HTML.jpg)
A phylogenetic tree of mesothelin precursor and related proteins. The unrooted tree was made using the Neighbor-Joining method [54] implemented in the MEGA3 program [55]. The tree is based on a multiple alignment from the MUSCLE program of the conserved regions of selected mesothelin, MPFL, stereocilin, and otoancorin proteins (see additional file 1 for the alignment). The number 0.2 on the scale bar indicates the number of substitutions per site.