Figure 7
From: (NZ)CH...O Contacts assist crystallization of a ParB-like nuclease
![Figure 7](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1472-6807-7-46/MediaObjects/12900_2007_Article_127_Fig7_HTML.jpg)
Process flow sheet of the crystallization strategy for the nuclease. The protein sample is purified to homogeneity using a combination of chromatographic methods. The pure and homogenous protein is chemically modified in order to localize the side chains and loops. The resultant compact protein molecule is then screened against a variety of chemical environments using commercially available sparse matrix screens to determine the best condition for the self-assembly of the protein molecules into a crystalline lattice.