| Tk MAT | Mj MAT | Ec MAT | Hs MAT |
---|---|---|---|---|
kinetic parameters | Â | Â | Â | Â |
V max [μmol/min/mg] | 1.95 ± 0.26 | 3.0 | 1.2 | 0.2 |
K M (Met) [mM] | 0.31 ± 0.07 | 0.14 | 0.08 | 0.003 |
K M (ATP) [mM] | 6.54 ± 2.49 | 0.25 | 0.11 | 0.03 |
assay | HPLC (SCX column) | radioactive filter binding assay | radioactive filter binding assay | radioactive filter binding assay |
buffer | 0.1 M Tris–HCl, 0.02 M MgCl2, 0.2 KCl, pH 8.0 | 50 mM K-Hepes, 10 mM MgCl2, 25 mM KCl, pH 8.0 | 0.1 Tris-Cl, 6 mM MgCl2, 0.1 KCl, pH 8.3 | 50 mM K-Tes, 50 mM KCl, 10 mM MgCl2, 10 mM DTT, 0.3 mM Na2EDTA, 0.1 mM BSA, pH 7.4 |
substrates | 10 mM l-methionine 10 mM ATP | 0.1 mM l-[methyl-14C]-methionine 5 mM ATP | 0.6 mM l-[methyl-14C]-methionine 4.4 mM 14C-ATP | 0.01 mM l-[methyl-14C]-methionine 5 mM ATP |
temperature | 37°C | 70°C | 25°C | 37°C |
reference | this work | Graham et al. [16] | Markham et al. [12] | Kotb & Kredich [25] |